-
1. Biuret test - Wikipedia
Link: https://en.wikipedia.org/wiki/Biuret_test
Description: WebBiuret reagent. The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate, the latter of which is added to chelate and thus stabilize the cupric ions. The reaction of the cupric ions with the nitrogen atoms involved in peptide bonds leads to the displacement of the peptide ...
-
2. Biuret Test: Principle, Reagent, Procedure &Result Interpretation
Link: https://biocheminsider.com/biuret-test/
Description: WebApr 9, 2021 · Objective Of Biuret Test. To detect the protein in the given solution. To analyze the presence of the peptide bond in a given analyte. Biuret Reagent. The Biuret reagent is a solution composed of sodium hydroxide (NaOH) or potassium hydroxide (KOH), hydrated copper (II) sulfate, and potassium sodium tartrate.
-
3. Biuret Test for Protein- Principle, Procedure, Results, Uses
Link: https://microbenotes.com/biuret-test-for-protein/
Description: WebApr 16, 2023 · These unshared electrons of the peptide bonds, in an alkaline environment, can be used by cupric ion (Cu +2) present in the Biuret reagent to form a violet or purple-colored complex. This colorimetric chemical test used to detect the peptide bond using the Biuret reagent is called the Biuret test.
-
4. Biuret Test - Checking for Peptide Bonds with Biuret Reagent
Link: https://byjus.com/chemistry/biuret-test/
Description: WebBiuret Reagent. The biuret reagent is made up of hydrated copper sulfate, sodium hydroxide, and Rochelle salt (sodium-potassium tartrate). Here, the Rochelle salt acts as a chelating agent and stabilizes the copper(II) ions. Biuret Test Principle. The copper(II) present in the biuret reagent binds itself to the nitrogen atoms that are present ...
-
5. Biuret Reaction - an overview | ScienceDirect Topics
Link: https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/biuret-reaction
Description: WebChapters and Articles. Quantification and Analysis of Proteins. Chang-Hui Shen, in Diagnostic Molecular Biology, 2019. Biuret Reaction. The biuret reaction is based on the complex formation of cupric ions with proteins. In this reaction, copper sulfate is added to a protein solution in strong alkaline solution.
-
6. Biuret Test: Principle, Procedure, and Uses • Microbe Online
Link: https://microbeonline.com/biuret-test-principle-procedure-and-uses/
Description: WebMay 10, 2023 · The biuret test is a colorimetric test that helps detect specific proteins or peptide bonds in given analytes. It is followed by spectrophotometry for quantification. The test requires the use of a biuret reagent. This reagent is a solution that consists of hydrated copper (II) sulfate, sodium hydroxide, and potassium sodium tartrate.
-
7. What does Biuret test for? Its Principle, Mechanism and Uses
Link: https://laboratoryinfo.com/biuret-test/
Description: WebMar 7, 2022 · What is biuret reagent? How to perform the biuret test for protein when using Fehling's A and B solutions? An indicator that protein is present is when the color changes to violet. Although the test is called biuret, it does not use the chemical biuret; a substance derived from urea. In fact, biuret is not a protein.
-
8. Biuret test - Definition and Examples - Biology Online Dictionary
Link: https://www.biologyonline.com/dictionary/biuret-test
Description: WebMar 3, 2023 · Biology definition: A Biuret test is used for detecting compounds with peptide bonds. A biuret reagent may be used to test the aqueous sample. Thus, the biuret test is used to detect proteins. That is because proteins are made up of polypeptides, which in turn, are made of amino acids joined by peptide bonds.
-
9. Biuret Test: Definition, Theory, Procedure, and Results
Link: https://www.chemistrylearner.com/biuret-test.html
Description: WebThe Biuret reagent is a solution that is made of sodium hydroxide (NaOH) or potassium hydroxide (KOH), hydrated copper (II) sulfate, and potassium sodium tartrate. The latter is added to chelate and thus stabilize the cupric ions. Both, NaOH and KOH provide the alkaline medium [3]. Procedure for Biuret Test [4, 5] Take 3 clean and dry test tubes.
-
10. Biuret Test - an overview | ScienceDirect Topics
Link: https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/biuret-test
Description: WebBiuret test is used for the detection of proteins with tyrosine and tryptophan amino acid in their composition. Phenylalanine does not give a positive result. Proteins having these amino acids give yellow color when heated with conc. HNO 3. The aromatic benzene ring undergoes nitration.