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1. Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe
aatbio.com
Link: https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-3-to-6-2
Description: Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe. Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium.
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2. Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe
aatbio.com
Link: https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6
Description: Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium. Available for purchase here.
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3. Citrate Buffer recipe - Sharebiology
sharebiology.com
Link: https://sharebiology.com/citrate-buffer-recipe/
Description: Citrate buffer is a universal buffer used in various reactions. It is sometimes combined with sodium citrate or completely replaced by sodium citrate buffer. However, alkalized citrate buffer has a similar composition to sodium citrate buffer.
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4. Simple Steps for Making Sodium Citrate Buffer - ThoughtCo
thoughtco.com
Link: https://www.thoughtco.com/how-to-make-sodium-citrate-buffer-375494
Description: Nov 30, 2019 · There are two methods of making sodium citrate buffers, depending on the materials accessible to you. If you have both citric acid and the conjugate base, create a stock solution of each by mixing 21 grams of citric acid in 1 liter of distilled water, and 29.4 grams of sodium citrate in 1 liter of distilled water.
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5. Buffer Reference Center - MilliporeSigma
sigmaaldrich.com
Link: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-concentration-and-buffer-exchange/buffer-reference-center
Description: Phosphate Buffer Preparation – 0.2 M solution. Citric Acid – Na 2 HPO 4 Buffer Preparation, pH 2.6-7.6. Citric Acid – Sodium Citrate Buffer Preparation, pH 3.0-6.2. Sodium Acetate – Acetic Acid Buffer Preparation, pH 3.7-5.6. Na 2 HPO 4 – NaH 2 …
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6. Citrate: How to Get Started and What, When, and How to Monitor?
nih.gov
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231307/
Description: Oct 9, 2018 · Treatment: replacement with buffer-free solution or the citrate is down titrated to half (= halving the dose of citrate) especially in the predilution system. If the cause is accidental or over-infusion of citrate: stop the citrate infusion, replace it with buffer-free solution, and increase filtration flow as necessary
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7. Citrate Buffer Solution, 0.09 M, C2488, Sigma-Aldrich
sigmaaldrich.com
Link: https://www.sigmaaldrich.com/US/en/product/SIGMA/C2488
Description: Mar 7, 2024 · Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues. [1]
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8. A guide for the preparation and use of buffers in biological …
unc.edu
Link: https://www.med.unc.edu/pharm/sondeklab/wp-content/uploads/sites/868/2018/10/buffers_calbiochem.pdf
Description: A word to our valued customers. We are pleased to present to you the newest edition of Buffers: A Guide for the Preparation and Use of Buffers in Biological Systems. This practical resource has been especially revamped for use by researchers in the biological sciences. citrate
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9. Citrate Buffer, pH 6.0, 10×, Antigen Retriever - MilliporeSigma
sigmaaldrich.com
Link: https://www.sigmaaldrich.com/US/en/product/sigma/c9999
Description: Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. [1] In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular ...
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10. Citrate Buffer - protocols.io
protocols.io
Link: https://www.protocols.io/view/citrate-buffer-n92ldyn28l5b/v1
Description: Abstract. A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues.
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11. Citrate Buffer Antigen Retrieval Protocol | Protocols Online
protocolsonline.com
Link: https://www.protocolsonline.com/histology/immunohistochemistry-histology/citrate-buffer-antigen-retrieval-protocol/
Description: Jul 18, 2012 · Citrate buffer antigen retrieval is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues [1-6]. When tissues are fixed in cross-linking agents such as paraformaldehyde, these agents will covalently cross-linked proteins, resulting a reduction in the available epitopes for antibody binding.
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12. Citric acid - Wikipedia
wikipedia.org
Link: https://en.wikipedia.org/wiki/Citric_acid
Description: Citric acid is an alpha hydroxy acid and is an active ingredient in chemical skin peels. [38] Citric acid is commonly used as a buffer to increase the solubility of brown heroin. [39] Citric acid is used as one of the active ingredients in the production of facial tissues with antiviral properties.
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13. Buffer Definition and Examples in Chemistry - Science Notes and …
sciencenotes.org
Link: https://sciencenotes.org/buffer-definition-and-examples-in-chemistry/
Description: Jan 21, 2024 · A buffer solution typically consists of a weak acid and its conjugate base, or a weak base and its conjugate acid. These components work in tandem to neutralize any added acid or base. Examples of Buffers. For example, the following acid and base pairs work together and form buffer solutions.
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14. 8.7: Buffer Solutions - Chemistry LibreTexts
libretexts.org
Link: https://chem.libretexts.org/Courses/Williams_School/Advanced_Chemistry/08%3A_Acids_and_Bases/8.07%3A_Buffer_Solutions
Description: Aug 20, 2021 · A buffer (or buffered) solution is one that resists a change in its pH when H + or OH – ions are added or removed owing to some other reaction taking place in the same solution. Buffers do so by being composed of certain pairs of solutes: either a weak acid plus its conjugate base or a weak base plus its conjugate acid.
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15. Heat-Induced Antigen Retrieval for Immunohistochemical Reactions …
nih.gov
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604828/
Description: Keywords: Heat-induced epitope retrieval, Archived tissues, Restoring epitope conformation, Paraffin sections, Time and temperature control, Microwave, Pressure cooker, Citrate buffer pH 6.0, Tris buffer pH 8.0, Tris–EDTA buffer pH 9.0. Go to: 1. Introduction. Immunohistochemistry has become an essential adjunct of modern diagnostic pathology.
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16. Citrate Buffer (pH 6.0), Concentrate - Thermo Fisher Scientific
thermofisher.com
Link: https://www.thermofisher.com/order/catalog/product/005000
Description: Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. During epitope retrieval, tissue slides are immersed.
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17. 0.1M Citric acid-Sodium citrate buffer buffer – pH range 3.0 – 6
mystrica.com
Link: http://www.mystrica.com/Pages/Applications/citratebuffer.pdf
Description: Document1. 0.1MCitricacidSodiumcitratebufferbuffer–pH range3.0–6.2. Preparea0.1Msolutionofcitricacidmonohydrate, C6H8O 7H 2O(21.01g/l)anda 0.1Msolutionoftrisodiumcitratedihydrate,C 6H5O 7Na32H2O(29.41g/l).Mixthe volumesshowninthetable. Ordissolvethemassesshownandmakeupto100cm 3withwater.
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18. 7.2: Practical Aspects of Buffers - Chemistry LibreTexts
libretexts.org
Link: https://chem.libretexts.org/Courses/Bellarmine_University/BU%3A_Chem_104_(Christianson)/Phase_2%3A_Understanding_Chemical_Reactions/7%3A_Buffer_Systems/7.2%3A_Practical_Aspects_of_Buffers
Description: Oct 24, 2023 · The buffer capacity is the amount of acid or base that can be added to a given volume of a buffer solution before the pH changes significantly, usually by one unit. Buffer capacity depends on the amounts of the weak acid and its … citrate
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19. 7.1: Acid-Base Buffers - Chemistry LibreTexts
libretexts.org
Link: https://chem.libretexts.org/Courses/Bellarmine_University/BU%3A_Chem_104_(Christianson)/Phase_2%3A_Understanding_Chemical_Reactions/7%3A_Buffer_Systems/7.1%3A_Acid-Base_Buffers
Description: Oct 23, 2023 · Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added (Figure \(\PageIndex{1}\)). A solution of acetic acid (\(\ce{CH3COOH}\) and sodium acetate \(\ce{CH3COONa}\)) is an example of … citrate
citrate -
20. Citric Acid: A Multifunctional Pharmaceutical Excipient - PMC
nih.gov
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148065/
Description: Apr 30, 2022 · This paper also analyzes the effect of citric acid in physiological processes and how this effect can be used to enhance the attributes of pharmaceutical preparations, as well as providing a critical discussion on the issues that may arise out …
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21. Structure and inhibition mechanism of the human citrate …
nature.com
Link: https://www.nature.com/articles/s41586-021-03230-x
Description: Feb 17, 2021 · Article 03 April 2019. Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Article. Structural insights into human organic cation transporter 1 transport and...
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22. Buffer Calculator - MilliporeSigma
sigmaaldrich.com
Link: https://www.sigmaaldrich.com/US/en/support/calculators-and-apps/buffer-calculator
Description: This buffer calculator provides an easy-to-use tool to calculate buffer molarity and prepare buffer solutions using the formula weight of the reagent and your desired volume (L, mL, or µL) and concentration (M, mM, or nM). To calculate the amount of buffer needed, please select a buffer from the Selection menu.
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23. Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe
aatbio.com
Link: https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-ph-5
Description: AAT Bioquest. Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe. A traditional buffer originally introduced in 1921. Since the Citrate-Phosphate buffer (also known as the McIlvaine buffer) only has 2 ingredients, the recipe can be adjusted to a pH range of 3-8.